UBE2I ELISA Kit

Catalog No. ABIN2044739

Product Details UBE2I ELISA Kit

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ubiquitin Conjugating Enzyme E2I (UBE2I) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2I (UBE2I), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Conjugating Enzyme E2I (UBE2I) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: Ubiquitin Conjugating Enzyme E2I (UBE2I) (UBE2I Products)

Synonyms: C358B7.1 ELISA Kit, P18 ELISA Kit, UBC9 ELISA Kit, ubc9 ELISA Kit, ube2ia ELISA Kit, zUbc9 ELISA Kit, 5830467E05Rik ELISA Kit, F830028O17Rik ELISA Kit, Ubce2i ELISA Kit, Ubce9 ELISA Kit, UbcE2A ELISA Kit, ubce9 ELISA Kit, T13J8.70 ELISA Kit, T13J8_70 ELISA Kit, UBIQUITIN-PROTEIN LIGASE ELISA Kit, ubiquitin conjugating enzyme 9 ELISA Kit, ubiquitin conjugating enzyme E2 I ELISA Kit, ubiquitin-conjugating enzyme E2Ib ELISA Kit, ubiquitin-conjugating enzyme E2L ELISA Kit, ubiquitin-conjugating enzyme E2I ELISA Kit, ubiquitin conjugating enzyme E2I S homeolog ELISA Kit, ubiquitin conjugating enzyme 9 ELISA Kit, SUMO-conjugating enzyme UBC9 ELISA Kit, UBE2I ELISA Kit, ube2ib ELISA Kit, UBE2L ELISA Kit, Ube2i ELISA Kit, ube2i.S ELISA Kit, UBC9 ELISA Kit, ubc-9 ELISA Kit, LOC108703134 ELISA Kit

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway