UBE2I ELISA Kit

Catalog No. ABIN5659734

Product Details UBE2I ELISA Kit

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2I (UBE2I). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2I (UBE2I) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2I (UBE2I), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Conjugating Enzyme E2I (UBE2I) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2I (UBE2I) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2I (UBE2I) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: Ubiquitin Conjugating Enzyme E2I (UBE2I Products)

Synonyms: C358B7.1 ELISA Kit, P18 ELISA Kit, UBC9 ELISA Kit, ubc9 ELISA Kit, ube2ia ELISA Kit, zUbc9 ELISA Kit, 5830467E05Rik ELISA Kit, F830028O17Rik ELISA Kit, Ubce2i ELISA Kit, Ubce9 ELISA Kit, UbcE2A ELISA Kit, ubce9 ELISA Kit, T13J8.70 ELISA Kit, T13J8_70 ELISA Kit, UBIQUITIN-PROTEIN LIGASE ELISA Kit, ubiquitin conjugating enzyme 9 ELISA Kit, ubiquitin conjugating enzyme E2 I ELISA Kit, ubiquitin-conjugating enzyme E2Ib ELISA Kit, ubiquitin-conjugating enzyme E2L ELISA Kit, ubiquitin-conjugating enzyme E2I ELISA Kit, ubiquitin conjugating enzyme E2I S homeolog ELISA Kit, ubiquitin conjugating enzyme 9 ELISA Kit, SUMO-conjugating enzyme UBC9 ELISA Kit, UBE2I ELISA Kit, ube2ib ELISA Kit, UBE2L ELISA Kit, Ube2i ELISA Kit, ube2i.S ELISA Kit, UBC9 ELISA Kit, ubc-9 ELISA Kit, LOC108703134 ELISA Kit

Background:

Gene Name: Ubiquitin Conjugating Enzyme E2I

Gene Aliases: P18, UBC9, UBCE9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, SUMO-conjugating enzyme UBC9

Gene ID: 7329

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway