C-JUN ELISA Kit

Catalog No. ABIN2044773

This Rat C-JUN ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat C-JUN.

Quick Overview for C-JUN ELISA Kit (ABIN2044773)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details C-JUN ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins