NF-kB p65 ELISA Kit

Catalog No. ABIN2044775

This Colorimetric ELISA kit is designed for the quantitative measurement of Human NF-kB p65.

Quick Overview for NF-kB p65 ELISA Kit (ABIN2044775)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details NF-kB p65 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins