VEGFR2/CD309 ELISA Kit
Quick Overview for VEGFR2/CD309 ELISA Kit (ABIN2044781)
Target
See all VEGFR2/CD309 (VEGFR2) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 1.56 ng/mL
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Sensitivity
- < 0.62 ng/mL
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VEGF Receptor 2 (VEGFR2) ELISA Kit
VEGFR2 Reactivity: Human AA 20-764 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 Reactivity: Mouse AA 20-762 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 Reactivity: Human Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Plate
- Pre-coated
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Protocol
- Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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Alternative Name
- Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
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Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
Target See all VEGFR2/CD309 (VEGFR2) ELISA Kits
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