CCL2 ELISA Kit

Catalog No. ABIN2112006

This Mouse CCL2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse CCL2.

Quick Overview for CCL2 ELISA Kit (ABIN2112006)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Product Details CCL2 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Brand: BioAssay™

Sensitivity: < 1.7 pg/mL

Components: MCP-1 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MCP-1 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Application Details

Plate: Pre-coated

Protocol: Elevated MCP-1 levels are associated with autoimmune disease, allergic inflammation, atherosclerosis, glomerulonephritis, granuloma formation. This ELISA kit provides a tool for studying MCP-1 expression and its relationship with various diseases in animal model.This mouse MCP-1 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse MCP-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse MCP-1, if present, will bind and become immobilized by the antibody pre-coated on the wells. A biotin conjugated antibody specific for mouse MCP-1 is added to each well. The biotin conjugated antibody will bind to the mouse MCP-1 on the plate. The microtiter plate wells are thoroughly washed to remove unbound biotin conjugate and other components of the sample. Avidin has a very high affinity to biotin. In order to quantify the amount of mouse MCP-1 present in the sample, a standardized preparation of avidin conjugated horseradish peroxidase (HRP) is added to each well. Avidin-HRPwill bind to the biotin on plate during incubation. The wells are thoroughly washed to remove all unbound Avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells to which mouse MCP-1, Biotin conjugate and Avidin-HRPare attached will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP-1

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection