IL-6 ELISA Kit

Catalog No. ABIN2648702

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat IL-6.

Quick Overview for IL-6 ELISA Kit (ABIN2648702)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Rat

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details IL-6 ELISA Kit

Purpose: Rat Interleukin 6 (IL-6) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of rat interleukin 6 (IL-6) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 7 pg/ml

Characteristics: Rat Interleukin 6 (IL-6) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: Rat Interleukin 6 (IL-6) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Interleukin 6 (IL-6) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interleukin 6 (IL-6) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Interleukin 6 (IL-6 )is added to the wells and binds to the combination of capture antibody-Rat interleukin 6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Interleukin 6 (IL-6) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Interleukin 6 (IL-6) standard dilutions and Interleukin 6 (IL-6) sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome