IL-6 ELISA Kit

Catalog No. ABIN411304

Mouse IL-6 ELISA Kit, Colorimetric assay for quantification of Mouse IL-6, that has been mentioned in 146+ publications.

Quick Overview for IL-6 ELISA Kit (ABIN411304)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Binding Specificity: AA 25-211

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Product Details IL-6 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Mouse IL-6/Interleukin-6 ELISA Kit to quantitate Mouse IL6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: F25-T211

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.7%, 4.9%, 7.2%
Inter-Assay Precision (CV%): 8.1%, 5.4%, 8.4%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IL-6 ELISA Kit (ABIN411304)

Cai, Chen, Zhong, Zhang, Jiang, Xu, Shi: "Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera." in: Scientific reports, Vol. 6, pp. 27014, (2018) (PubMed).

Liu, Xu, Chang, Qin, Yin, Yang: "4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-κB signaling in a ROS independent manner." in: Oncotarget, Vol. 7, Issue 27, pp. 40978-40991, (2018) (PubMed).

Lin, Chen, Xia, Yang: "MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3." in: Oncotarget, Vol. 7, Issue 31, pp. 48978-48994, (2018) (PubMed).

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Pang, Wu, Peng, Yang, Kuai, Qin, Cao, Sun, Chen, Vitek, Jiang: "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity." in: Oncotarget, Vol. 7, Issue 35, pp. 56030-56044, (2018) (PubMed).

Dong, Liu, Meng, Liu, Bi, Wu, Jin, Yao, Tang, Wang, Li, Zhang, Yu, Zhan, Chen, Ge, Yang, Li: "Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination." in: Scientific reports, Vol. 6, pp. 32710, (2018) (PubMed).

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Yang, Lai, Zheng, Kang, Xian, Wang, Shi, Meng, Li, Chen, Zhang: "A novel pH-controlled hydrogen sulfide donor protects gastric mucosa from aspirin-induced injury." in: Journal of cellular and molecular medicine, Vol. 21, Issue 10, pp. 2441-2451, (2018) (PubMed).

Wang, Zhang, Yang, Zou, Wang, Liu, Liu, Zhang, Xiao, Wang: "Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1." in: International journal of molecular medicine, Vol. 40, Issue 2, pp. 427-437, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." in: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Li, Diao, Guo, Huang, Yang, Feng, Yan, Ning, Zheng, Chen, Wu: "VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism." in: Nature communications, Vol. 8, Issue 1, pp. 1322, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Wang, Chen, Ni, Cheng, Jia, Zhen: "miRNA-3473b contributes to neuroinflammation following cerebral ischemia." in: Cell death & disease, Vol. 9, Issue 1, pp. 11, (2018) (PubMed).

Zhang, Feng, Cui, Yang, Zhu et al.: "Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box ..." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5805-5813, (2018) (PubMed).

Lu, Zhang, Zhang, Zhou, Gao, Liu, Wang, Wu, Li, Hang: "Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 87, (2018) (PubMed).

Zhu, Li, Zhang, Ding, Zhu, Huang, Zhang, Jia, Li: "COX-2 contributes to LPS-induced Stat3 activation and IL-6 production in microglial cells." in: American journal of translational research, Vol. 10, Issue 3, pp. 966-974, (2018) (PubMed).

Xu, Liu, Wang, Li, Wu, Li, Di, You, Jiang et al.: "3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress ..." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3271617, (2018) (PubMed).

Li, Fan, Xia, Xiao, Wang, Sun, Zhang, He: "The immunosuppressive characteristics of FB1 by inhibition of maturation and function of BMDCs." in: International immunopharmacology, Vol. 47, pp. 206-211, (2017) (PubMed).

Bai, Yin, Feng, Cao, Wu, Zhu, Li, Tu, Chai: "Corydalis hendersonii Hemsl. protects against myocardial injury by attenuating inflammation and fibrosis via NF-κB and JAK2-STAT3 signaling pathways." in: Journal of ethnopharmacology, Vol. 207, pp. 174-183, (2017) (PubMed).

Qu, Zhang, Cai, Li, Ma, Xu, Lu: "Exosomes derived from miR-181-5p-modified adipose-derived mesenchymal stem cells prevent liver fibrosis via autophagy activation." in: Journal of cellular and molecular medicine, (2017) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background:

Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) (interleukin-6), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8 kDa.

Gene Full Name: interleukin 6

Gene ID: 16193

UniProt: P08505

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome