IL12 ELISA Kit
Quick Overview for IL12 ELISA Kit (ABIN2648768)
Target
See all IL12 ELISA KitsReactivity
Detection Method
Application
Sample Type
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Purpose
- Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 12 (IL-12p70) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensitivity
- 7 pg/ml
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Characteristics
- Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 12 (IL12) ELISA Kit
IL12 Reactivity: Mouse Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rabbit Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Chicken Colorimetric Competition ELISA 0.78 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitSmall Sample IL12 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitHigh Sensitivity IL12 Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitSmall Sample IL12 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 3.5 h
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Plate
- Pre-coated
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Protocol
- Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p70 is added to the wells and binds to the combination of capture antibody- IL-12p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p70 standard dilutions and IL-12p70 sample concentration determined.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IL12 (Interleukin 12 (IL12))
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Alternative Name
- IL-12p70
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Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target See all IL12 ELISA Kits
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