IL12 ELISA Kit
Quick Overview for IL12 ELISA Kit (ABIN2648769)
Target
See all IL12 ELISA KitsReactivity
Detection Method
Application
Sample Type
-
-
Purpose
- Human Interleukin 12p70 (IL-12p70) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 12p70 (IL-12p70) concentrations in cell culture supernates, serum, and plasma.
-
Analytical Method
- Quantitative
-
Sensitivity
- 7 pg/ml
-
Characteristics
- Human Interleukin 12p70 (IL-12p70) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
-
-
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Sample Volume
- 100 μL
-
Assay Time
- 3.5 h
-
Plate
- Pre-coated
-
Protocol
- Human Interleukin 12p70 (IL-12p70) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p70 is added to the wells and binds to the combination of capture antibody-IL-12p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p70 standard dilutions and IL-12p70 sample concentration determined
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
-
- IL12 (Interleukin 12 (IL12))
-
Alternative Name
- IL-12p70
-
Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target See all IL12 ELISA Kits
-