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IL12 ELISA Kit

This Human IL12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL12.
Catalog No. ABIN2648769

Quick Overview for IL12 ELISA Kit (ABIN2648769)

Target

See all IL12 ELISA Kits
IL12 (Interleukin 12 (IL12))

Reactivity

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Human

Detection Method

Colorimetric

Application

ELISA

Sample Type

Serum, Plasma, Cell Culture Cells
  • Purpose

    Human Interleukin 12p70 (IL-12p70) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 12p70 (IL-12p70) concentrations in cell culture supernates, serum, and plasma.

    Analytical Method

    Quantitative

    Sensitivity

    7 pg/ml

    Characteristics

    Human Interleukin 12p70 (IL-12p70) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Sample Volume

    100 μL

    Assay Time

    3.5 h

    Plate

    Pre-coated

    Protocol

    Human Interleukin 12p70 (IL-12p70) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p70 is added to the wells and binds to the combination of capture antibody-IL-12p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p70 standard dilutions and IL-12p70 sample concentration determined

    Restrictions

    For Research Use only
  • Storage

    4 °C
  • Target See all IL12 ELISA Kits

    IL12 (Interleukin 12 (IL12))

    Alternative Name

    IL-12p70

    Pathways

    JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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