TNF alpha ELISA Kit

Catalog No. ABIN2648838

This Rat TNF alpha ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat TNF alpha.

Quick Overview for TNF alpha ELISA Kit (ABIN2648838)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Rat

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details TNF alpha ELISA Kit

Purpose: Rat TNF Alpha (TNF-α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of rat γ-interferon (TNF-α ) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 15 pg/ml

Characteristics: Rat TNF Alpha (TNF-α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Rat TNF Alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF Alpha (TNF-α) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF Alpha (TNF-α) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF Alpha (TNF-α) is added to the wells and binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF Alpha (TNF-α) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF Alpha (TNF-α) standard dilutions and TNF Alpha (TNF-α) sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome