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Mitochondrial ΔPsi drives the accumulation in mitochondria of cationic dyes such as cyanines, and the mitochondrial ΔPsi is reduced when energy metabolism is disrupted, notably in apoptosis. Changes in the mitochondrial ΔPsi have been described during necrosis, cell cycle and apoptosis. Mitochondrial uptake of dye is a possible source of fluorescence variance.Samples were tested on Flow Cytometry Samples can be run up to 3 hours after lysis.