C5A ELISA Kit
(4 references)Quick Overview for C5A ELISA Kit (ABIN2690553)
Target
See all C5A (C5a) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Minimum Detection Limit
- 0.047 ng/mL
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Purpose
- The Human C5a ELISA Kit II is for the in vitro quantitative determination of Human C5a-desArg in human EDTA plasma, serum and other biological samples.
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Analytical Method
- Quantitative
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Components
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Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each
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Complement Component 5a (C5a) ELISA Kit
C5a Reactivity: Human Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Mouse Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Cow Colorimetric Sandwich ELISA 4.69 ng/mL - 300 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rabbit Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Monkey Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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BD OptEIA™ Human C5a ELISA Kit II
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
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1. Store kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).
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: "Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation." in: Clinical chemistry, Vol. 45, Issue 8 Pt 1, pp. 1190-9, (1999) (PubMed).
: "Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease." in: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1497-502, (1993) (PubMed).
: "Anaphylatoxins: C3a and C5a." in: Advances in immunology, Vol. 26, pp. 1-53, (1978) (PubMed).
: "Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase." in: The Journal of clinical investigation, Vol. 49, Issue 12, pp. 2427-36, (1971) (PubMed).
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- C5A (C5a) (Complement Component 5a (C5a))
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Alternative Name
- C5a
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Pathways
- Complement System, Carbohydrate Homeostasis
Target See all C5A (C5a) ELISA Kits
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