IL-2 ELISA Kit

Catalog No. ABIN2690564

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-2. There are 5 publications available.

Quick Overview for IL-2 ELISA Kit (ABIN2690564)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Supernatant

Product Details IL-2 ELISA Kit

Purpose: The Human IL-2 ELISA Kit II is for the quantitative determination of human IL-2 in serum, plasma, and cell culture supernatant.

Brand: BD OptEIA™

Analytical Method: Quantitative

Application Details

Application Notes: The ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance "Recovery" and "Linearity" sections.

Comment:

BD OptEIA™ Human IL-2 ELISA Kit II

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for IL-2 ELISA Kit (ABIN2690564)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Smith: "Interleukin-2: inception, impact, and implications." in: Science (New York, N.Y.), Vol. 240, Issue 4856, pp. 1169-76, (1988) (PubMed).

Stern, Pan, Urdal, Mochizuki, DeChiara, Blacher, Wideman, Gillis: "Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 3, pp. 871-5, (1984) (PubMed).

Taniguchi, Matsui, Fujita, Takaoka, Kashima, Yoshimoto, Hamuro: "Structure and expression of a cloned cDNA for human interleukin-2." in: Nature, Vol. 302, Issue 5906, pp. 305-10, (1983) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: Interleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multi-functional cytokine that can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages. IL-2 exerts its biological effects by binding to specific receptors expressed by various target cells. Human IL-2 is a 15 kDa protein containing 133 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation