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Growth Hormone Receptor ELISA Kit

GHR Reactivity: Human Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2703050
$480.70
Plus shipping costs $45.00
Shipping to: United States
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    Growth Hormone Receptor (GHR)
    Reactivity
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.4-100 ng/mL
    Minimum Detection Limit
    0.4 ng/mL
    Application
    ELISA
    Purpose
    Human Growth Hormone R ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes human Growth Hormone R. Other species not yet determined.
    Sensitivity
    0.4 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling GHR ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
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    Growth Hormone Receptor (GHR)
    Alternative Name
    GHR (GHR Products)
    Synonyms
    GHBP, AA986417, GHR/BP, GHR, ghr, ghr.a, zgc:162141, growth hormone receptor, growth hormone receptor L homeolog, growth hormone receptor a, GHR, Ghr, ghr.L, ghr, ghra
    Background
    Gene Names: GHR
    Protein names: Growth hormone receptor (GH receptor) (Somatotropin receptor) [Cleaved into: Growth hormone-binding protein (GH-binding protein) (GHBP) (Serum-binding protein)]
    Gene ID
    2690
    UniProt
    P10912
    Pathways
    NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus
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