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GM-CSF ELISA Kit

CSF2 Reactivity: Pig Colorimetric Sandwich ELISA 72-18.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2703063
$480.70
Plus shipping costs $45.00
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  • Target See all GM-CSF (CSF2) ELISA Kits
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Reactivity
    • 14
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Pig
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    72-18.000 pg/mL
    Minimum Detection Limit
    72 pg/mL
    Application
    ELISA
    Purpose
    Porcine GM-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-gamma, TGF beta 1, TNF alpha
    Sensitivity
    72 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling CSF2 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all GM-CSF (CSF2) ELISA Kits
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Alternative Name
    GM-CSF (CSF2 Products)
    Synonyms
    GMCSF, GM-CSF, CSF2, Csfgm, Gm-CSf, MGI-IGM, Gm-csf, Gmcsf, CSF, colony stimulating factor 2, colony stimulating factor 2 (granulocyte-macrophage), CSF2, Csf2
    Background
    Gene Names: CSF2
    Protein names: Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF)
    Gene ID
    397208
    UniProt
    Q29118
    Pathways
    JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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