IL12 ELISA Kit
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- Target See all IL12 ELISA Kits
- IL12 (Interleukin 12 (IL12))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 5-2000 pg/mL
- Minimum Detection Limit
- 5 pg/mL
- Application
- ELISA
- Purpose
- Mouse IL-12 p40/p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
- Sensitivity
- 5 pg/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product IL12 ELISA Kit
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- Application Notes
- Recommended Dilution for serum and plasma samples10 - 100 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." in: Journal of neurochemistry, (2015) (PubMed).
: "Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 27, Issue 3, pp. 347-55, (2015) (PubMed).
: "Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine." in: Cellular signalling, Vol. 27, Issue 7, pp. 1398-412, (2015) (PubMed).
: "Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophages and possible mechanisms of CCR-2/TLR-2 and MCP-1 signalling during Staphylococcus aureus infection." in: Immunobiology, Vol. 220, Issue 3, pp. 350-62, (2015) (PubMed).
: "Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes." in: Frontiers in pharmacology, Vol. 6, pp. 56, (2015) (PubMed).
: "Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes." in: Frontiers in pharmacology, Vol. 6, pp. 74, (2015) (PubMed).
: "Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling." in: Blood, Vol. 121, Issue 6, pp. 930-9, (2013) (PubMed).
: "Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis." in: Experimental dermatology, Vol. 21, Issue 9, pp. 714-5, (2012) (PubMed).
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Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." in: Journal of neurochemistry, (2015) (PubMed).
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- Target See all IL12 ELISA Kits
- IL12 (Interleukin 12 (IL12))
- Alternative Name
- IL-12 p40/70 (IL12 Products)
- Background
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Gene Names: Il12b
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
- Gene ID
- 16160
- UniProt
- P43432
- Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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