IL-25 ELISA Kit

Catalog No. ABIN2703183

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-25. There is 1 publication available.

Quick Overview for IL-25 ELISA Kit (ABIN2703183)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.4 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL-25 ELISA Kit

Minimum Detection Limit: 0.4 ng/mL

Purpose: Human IL-17E (IL-25) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen, B7-H1, BMPR-IA, BMPR-II, Cadherin-11, CD27, CD6, Ck beta 8-1 , CNTF, DNAM-1, EMMPRIN, FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand, Granulysin, IL-1 R3, IL-15 R, IL-32 alpha, L1CAM-2, LRIG3, LRP-6, MEPE, Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100A8, Siglec-7, Syndecan-3, Thrombospondin-2, Thrombospondin-5, Tie-1, ULBP-2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for IL-25 ELISA Kit (ABIN2703183)

Wang, Fan, Lv, Yao, Wang, Huang, Meng, Fang, Lee, Corrigan, An, Ying: "Interleukin-25 promotes basic fibroblast growth factor expression by human endothelial cells through interaction with IL-17RB, but not IL-17RA." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 42, Issue 11, pp. 1604-14, (2013) (PubMed).

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL-17 E

Background:

Gene Symbols: IL25, IL17E, UNQ3120/PRO10272

Protein Name (Synonyms): Interleukin-25 (IL-25) (Interleukin-17E) (IL-17E)

Gene ID: 64806

UniProt: Q9H293