SMAD2 ELISA Kit (SMAD, Mothers Against DPP Homolog 2)

Details for Product SMAD2 ELISA Kit No. ABIN2747901
Antigen
  • JV18
  • JV18-1
  • MADH2
  • MADR2
  • hMAD-2
  • hSMAD2
  • jv18
  • Xmad2
  • madh2
  • madr2
  • XSmad2
  • hmad-2
  • hsmad2
  • jv18-1
  • smad-2
  • fj43c06
  • wu:fj43c06
  • 7120426M23Rik
  • Madh2
  • Madr2
  • Smad-2
  • mMad2
  • MAD homolog 2
  • Mothers against DPP homolog 2
  • SMAD 2
  • Smad2
  • SMAD family member 2
  • SMAD family member 2-Z
  • SMAD family member 2 S homeolog
  • SMAD2
  • SMAD2Z
  • smad2
  • smad2.S
  • Smad2
Epitope
pSer255, pSer250, pSer245, total
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
9
6
5
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) and Total SMAD2 Kit. This assay semi-quantitatively measures phophorylated SMAD2 (Ser245/250/255) and Total SMAD2 in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes human, mouse, rat SMAD2 phosphorylated at site Serine-245/250/255 and Total SMAD2.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others SMAD2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name SMAD2 (SMAD2 ELISA Kit Abstract)
Background Mothers against decapentaplegic homolog 2 (SMAD2) phosphorylated at Serine-245/250/255 and Total SMAD2
Gene ID 4087
UniProt Q15796
Pathways Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
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