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Reticulon 4 ELISA Kit

RTN4 Reactivity: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN2748436
  • Target See all Reticulon 4 (RTN4) ELISA Kits
    Reticulon 4 (RTN4)
    Reactivity
    • 4
    • 2
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Rat Nogo-A (Reticulon 4A) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Rat Nogo-A. Other species not yet determined.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Reticulon 4 (RTN4) ELISA Kits
    Reticulon 4 (RTN4)
    Alternative Name
    Nogo-A (RTN4 Products)
    Synonyms
    ASY ELISA Kit, NI220/250 ELISA Kit, NOGO ELISA Kit, NOGO-A ELISA Kit, NOGOC ELISA Kit, NSP ELISA Kit, NSP-CL ELISA Kit, Nbla00271 ELISA Kit, Nbla10545 ELISA Kit, Nogo-B ELISA Kit, Nogo-C ELISA Kit, RTN-X ELISA Kit, RTN4-A ELISA Kit, RTN4-B1 ELISA Kit, RTN4-B2 ELISA Kit, RTN4-C ELISA Kit, RTN4-Cw ELISA Kit, DKFZp459C0314 ELISA Kit, 1110020G17Rik ELISA Kit, AA407876 ELISA Kit, AA409940 ELISA Kit, AA960376 ELISA Kit, C130026I10Rik ELISA Kit, NgA ELISA Kit, Nogo-A ELISA Kit, mKIAA0886 ELISA Kit, mKIAA4153 ELISA Kit, NI-250 ELISA Kit, Nogo ELISA Kit, Vp20 ELISA Kit, r ELISA Kit, rat N ELISA Kit, rat NogoA ELISA Kit, reticulon-4 ELISA Kit, nogo ELISA Kit, rtn4 ELISA Kit, wu:fb17a02 ELISA Kit, wu:fb22f12 ELISA Kit, wu:fd61a07 ELISA Kit, zgc:92163 ELISA Kit, reticulon 4 ELISA Kit, reticulon 4a ELISA Kit, RTN4 ELISA Kit, rtn4 ELISA Kit, Rtn4 ELISA Kit, rtn4a ELISA Kit
    Background
    Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein)
    Gene ID
    83765
    UniProt
    Q9JK11
    Pathways
    Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome
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