Leptin ELISA Kit

Catalog No. ABIN2859273

The Mouse Leptin ELISA Kit (ABIN2859273) is a Colorimetric ELISA Kit designed to quantify Mouse Leptin. This ELISA Kit has been cited in 12 publications.

Quick Overview for Leptin ELISA Kit (ABIN2859273)

Target: Leptin (LEP)

Binding Specificity: AA 22-167

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details Leptin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich Mouse Leptin ELISA Kit to quantitate Mouse Lep in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: V22-C167

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7%, 4.9%, 7%
Inter-Assay Precision (CV%): 7.6%, 4.9%, 8.4%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for Leptin ELISA Kit (ABIN2859273)

Lee, Lee, Chung, Nam, Kim, Park, Hong, Yang, Kim, Jeong: "Beneficial Effects of Red Yeast Rice on High-Fat Diet-Induced Obesity, Hyperlipidemia, and Fatty Liver in Mice." in: Journal of medicinal food, Vol. 18, Issue 10, pp. 1095-102, (2015) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

Yuan, Huang, Li, Zhang, Li, Li, Gao, Zeng, Shan, Lin, Huang: "Hyperleptinemia directly affects testicular maturation at different sexual stages in mice, and suppressor of cytokine signaling 3 is involved in this process." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 15, (2014) (PubMed).

Lee, Nam, Chung, Kim, Park, Chung, Kim, Sohn, Kim, Oh, Jeong: "Beneficial effects of phosphatidylcholine on high-fat diet-induced obesity, hyperlipidemia and fatty liver in mice." in: Life sciences, Vol. 118, Issue 1, pp. 7-14, (2014) (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

Hajihashemi, Azadbakht, Hashemipor, Kelishadi, Esmaillzadeh: "Whole-grain intake favorably affects markers of systemic inflammation in obese children: a randomized controlled crossover clinical trial." in: Molecular nutrition & food research, Vol. 58, Issue 6, pp. 1301-8, (2014) (PubMed).

Yang, Zhang, Fu, Yang: "Leptin and IL-8: two novel cytokines screened out in childhood lead exposure." in: Toxicology letters, Vol. 227, Issue 3, pp. 172-8, (2014) (PubMed).

Karadag, Ertugrul, Kalkan, Bilgili, Celik, Takci, Balahoroglu, Calka: "The effect of acitretin treatment on insulin resistance, retinol-binding protein-4, leptin, and adiponectin in psoriasis vulgaris: a noncontrolled study." in: Dermatology (Basel, Switzerland), Vol. 227, Issue 2, pp. 103-8, (2013) (PubMed).

Xie, Yang, Zou, Cheng, Wang, Jiang, Xiao, Wang, Liu: "Influence of the core circadian gene "Clock" on obesity and leptin resistance in mice." in: Brain research, Vol. 1491, pp. 147-55, (2012) (PubMed).

Yao, Li, Zhao, Zeng, Shen, Xiang, Xiao: "Anti-obesity effect of an isoflavone fatty acid ester on obese mice induced by high fat diet and its potential mechanism." in: Lipids in health and disease, Vol. 9, pp. 49, (2010) (PubMed).

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: LEP

Background:

Background: Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83 % homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

Gene Full Name: leptin

Gene ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process