Angiotensin II FLISA Kit
Quick Overview for Angiotensin II FLISA Kit (ABIN2871086)
Target
See all Angiotensin II (Ang II) FLISA KitsReactivity
Detection Method
Method Type
Application
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Analytical Method
- Quantitative
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Angiotensin II (Ang II) ELISA Kit
Ang II Reactivity: Mouse Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Rat Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Human Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Rat Colorimetric Sandwich ELISA 4.7 pg/mL - 300 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Rabbit Colorimetric Competition ELISA 4.94 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Chicken Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Cow Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Horse Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II Reactivity: Dog Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Angiotensin II (Ang II)
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Alternative Name
- Angiotensin II
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Pathways
- JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
Target See all Angiotensin II (Ang II) FLISA Kits
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