Factor VIII ELISA Kit

Catalog No. ABIN2946438

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Factor VIII.

Quick Overview for Factor VIII ELISA Kit (ABIN2946438)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma

Product Details Factor VIII ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Analytical Method: Quantitative

Sensitivity: 0.1 ng/mL

Components: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Material not included:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Application Details

Plate: Pre-coated

Assay Procedure:

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• 1) Set standard, test samples and control wells.

• 2) Aliquot 100 μL of diluted standard into the standard wells.

• 3) Aliquot 100 μL of Standard Diluent buffer into control (zero) well.

• 4) Aliquot 100 μL of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.

• 5) Aliquot 100 μL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.

• 6) Wash 3 times.

• 7) Aliquot 100 μL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.

• 8) Wash 5 times.

• 9) Aliquot 90 μL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.

• 10) Aliquot 50 μL of Stop Solution.

• 11) Measure the OD at 450 nm.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Coagulation Factor VIII were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Coagulation Factor VIII were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Expiry Date: 6 months

Target Details for Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Alternative Name: Coagulation Factor VIII