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Caspase 3 ELISA Kit

CASP3 Reactivity: Human AA 183-277 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate
Catalog No. ABIN3044699
  • Target See all Caspase 3 (CASP3) ELISA Kits
    Caspase 3 (CASP3)
    Binding Specificity
    AA 29-175, AA 183-277
    Reactivity
    • 8
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Caspase 3
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: S29-D175&A183-H277
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Immunogen sequence: S29-D175&A183-H277
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

    Plate
    Pre-coated
    Protocol
    human Caspase 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Caspase 3 has been precoated onto 96-well plates. Standards(Expression system for standard: E. coli, Immunogen sequence: S29-D175 & A183-H277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Caspase 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Caspase 3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Caspase 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Caspase 3 standard solution and each sample be measured in duplicate.

    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all Caspase 3 (CASP3) ELISA Kits
    Caspase 3 (CASP3)
    Alternative Name
    CASP3 (CASP3 Products)
    Synonyms
    CPP32 ELISA Kit, CPP32B ELISA Kit, SCA-1 ELISA Kit, A830040C14Rik ELISA Kit, AC-3 ELISA Kit, Apopain ELISA Kit, CC3 ELISA Kit, Caspase-3 ELISA Kit, Lice ELISA Kit, Yama ELISA Kit, mldy ELISA Kit, xcpp32 ELISA Kit, casp3 ELISA Kit, zgc:100890 ELISA Kit, CASP-3 ELISA Kit, caspase-3 ELISA Kit, caspase 3 ELISA Kit, caspase 3 S homeolog ELISA Kit, caspase 3, apoptosis-related cysteine peptidase a ELISA Kit, caspase 3, apoptosis-related cysteine peptidase ELISA Kit, CASP3 ELISA Kit, Casp3 ELISA Kit, casp3.S ELISA Kit, casp3a ELISA Kit
    Background

    Protein Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. .

    Background: Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

    Synonyms: Caspase-3,CASP-3,3.4.22.56,Apopain,Cysteine protease CPP32,CPP-32,Protein Yama,SREBP cleavage activity 1,SCA-1,Caspase-3 subunit p17,Caspase-3 subunit p12,CASP3,CPP32,

    Full Gene Name: Caspase-3

    Cellular Localisation: Cytoplasm.
    Gene ID
    836
    UniProt
    P42574
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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