IL6RA ELISA Kit

Catalog No. ABIN3198462

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL6RA.

Quick Overview for IL6RA ELISA Kit (ABIN3198462)

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.63 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL6RA ELISA Kit

Minimum Detection Limit: 15.63 pg/mL

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytical Method: Quantitative

Specificity: IL-6 Rα ELISA Kit, Human

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for IL6RA

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Alternative Name: IL-6 Ralpha

Background: Interleukin 6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6 Rα) and interleukin 6 signal transducer Glycoprotein 130 (gp130). A soluble form of the IL-6 Rα with a molecular weight of approximately 50 kDa apparently arises from proteolytic cleavage of membrane-bound IL-6 Rα.

Gene ID: 3570

NCBI Accession: NM_000565

UniProt: P08887

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints