CD14 ELISA Kit

Catalog No. ABIN3198513

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CD14.

Quick Overview for CD14 ELISA Kit (ABIN3198513)

Target: CD14

Binding Specificity: Soluble

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details CD14 ELISA Kit

Minimum Detection Limit: 125 pg/mL

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytical Method: Quantitative

Specificity: SCD14 ELISA Kit, Human

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is expressed mainly by macrophages and (at 10-times lesser extent) by neutrophils. It is also expressed by dendritic cells. It is a component of the innate immune system. CD14 acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). sCD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted.

Gene ID: 929

NCBI Accession: NM_001174105

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades