BDNF ELISA Kit

Catalog No. ABIN411258

This Rat BDNF ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat BDNF. This ELISA Kit has been cited in 16+ publications.

Quick Overview for BDNF ELISA Kit (ABIN411258)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Binding Specificity: AA 129-247

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Product Details BDNF ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat BDNF

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: sf21
Immunogen sequence: H129-R247

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Predicted Reactivity: Bovine,Canine,Monkey,Rabbit

Sensitivity: <2pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: H129-R247

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BDNF has been precoated onto 96-well plates. Standards(sf21, H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BDNF amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat BDNF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat BDNF standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 109, Standard deviation: 3.60, CV(%): 3.3
• Sample 2: n=16, Mean(pg/ml): 447, Standard deviation: 17.43, CV(%): 3.9
• Sample 3: n=16, Mean(pg/ml): 1195, Standard deviation: 51.39, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 8.35, CV(%): 6.9
• Sample 2: n=24, Mean(pg/ml): 468, Standard deviation: 33.23, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 1257, Standard deviation: 90.5, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for BDNF ELISA Kit (ABIN411258)

Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

Sun, Zhu, Yin, Guo, Xu, Xiao, Jiang, Guo, Meng, Lu, Wang, Peng: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).

Hibicke, Graham, Hayslett: "Adolescent chronic restraint stress (aCRS) elicits robust depressive-like behavior in freely cycling, adult female rats without increasing anxiety-like behaviors." in: Experimental and clinical psychopharmacology, Vol. 25, Issue 2, pp. 74-83, (2017) (PubMed).

Cai, Wu, Huang, Huang, Chen, Wang: "Effect of Zhuang Jing Decoction on Learning and Memory Ability in Aging Rats." in: Rejuvenation research, Vol. 19, Issue 4, pp. 303-8, (2017) (PubMed).

Liu, Zhang, Rong, Li, Wang: "Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron-astrocyte co-culture system through neurotrophy and neuroinflammation pathway." in: Acta pharmaceutica Sinica. B, Vol. 7, Issue 5, pp. 554-563, (2017) (PubMed).

Cheng, Li, Zhao, Yu, Yang, Wang, Chen, Yang: "Short-term sleep deprivation stimulates hippocampal neurogenesis in rats following global cerebral ischemia/reperfusion." in: PLoS ONE, Vol. 10, Issue 6, pp. e0125877, (2016) (PubMed).

Chung, Chung, Lee, Chung, Yang, Lee, Choi, Chang, Kim, Suh, Lee, Hwang, Do, Kim: "Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats." in: Journal of veterinary science, Vol. 17, Issue 1, pp. 97-102, (2016) (PubMed).

Azman, Zakaria, Abdul Aziz, Othman: "Tualang Honey Attenuates Noise Stress-Induced Memory Deficits in Aged Rats." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1549158, (2016) (PubMed).

Dayi, Cetin, Sisman, Aksu, Tas, Gönenc, Uysal: "The effects of oxytocin on cognitive defect caused by chronic restraint stress applied to adolescent rats and on hippocampal VEGF and BDNF levels." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 69-75, (2015) (PubMed).

Sisman, Kiray, Camsari, Evren, Ates, Baykara, Aksu, Guvendi, Uysal: "Potential novel biomarkers for diabetic testicular damage in streptozotocin-induced diabetic rats: nerve growth factor Beta and vascular endothelial growth factor." in: Disease markers, Vol. 2014, pp. 108106, (2014) (PubMed).

Uysal, Kiray, Sisman, Camsari, Gencoglu, Baykara, Cetinkaya, Aksu: "Effects of voluntary and involuntary exercise on cognitive functions, and VEGF and BDNF levels in adolescent rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 90, Issue 1, pp. 55-68, (2014) (PubMed).

Kranz, Warnecke, Lenarz, Durisin, Scheper: "Phosphodiesterase type 4 inhibitor rolipram improves survival of spiral ganglion neurons in vitro." in: PLoS ONE, Vol. 9, Issue 3, pp. e92157, (2014) (PubMed).

Uysal, Sisman, Dayi, Ozbal, Cetin, Baykara, Aksu, Tas, Cavus, Gonenc-Arda, Buyuk: "Acute footshock-stress increases spatial learning-memory and correlates to increased hippocampal BDNF and VEGF and cell numbers in adolescent male and female rats." in: Neuroscience letters, Vol. 514, Issue 2, pp. 141-6, (2012) (PubMed).

Aksu, Baykara, Ozbal, Cetin, Sisman, Dayi, Gencoglu, Tas, Büyük, Gonenc-Arda, Uysal: "Maternal treadmill exercise during pregnancy decreases anxiety and increases prefrontal cortex VEGF and BDNF levels of rat pups in early and late periods of life." in: Neuroscience letters, Vol. 516, Issue 2, pp. 221-5, (2012) (PubMed).

Uysal, Sisman, Dayi, Aksu, Cetin, Gencoglu, Tas, Buyuk: "Maternal exercise decreases maternal deprivation induced anxiety of pups and correlates to increased prefrontal cortex BDNF and VEGF." in: Neuroscience letters, Vol. 505, Issue 3, pp. 273-8, (2011) (PubMed).

Kobayashi, Yamagishi, Kamamoto, Yoshida, Uchino: "Effect of hyperthermia on hemopoietic stem cells increased thermal sensitivity along the stem cell differentiation pathway." in: Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society, Vol. 48, Issue 1, pp. 37-46, (1985) (PubMed).

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background:

Protein Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS (By similarity). .

Background: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8 kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.

Synonyms: Brain-derived neurotrophic factor,BDNF,Bdnf,

Full Gene Name: Brain-derived neurotrophic factor

Cellular Localisation: Secreted.

Gene ID: 24225

UniProt: P23363

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity