EGFR ELISA Kit

Catalog No. ABIN411266

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EGFR. There are 4 publications available.

Quick Overview for EGFR ELISA Kit (ABIN411266)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Binding Specificity: AA 1-645

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk

Product Details EGFR ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human soluble EGFR ELISA Kit to quantitate Human EGFR in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: M1-S645

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.7%, 5%, 7.4%
Inter-Assay Precision (CV%): 6%, 5.9%, 7.5%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for EGFR ELISA Kit (ABIN411266)

Zhang, Mao, Zhang, Ye, Tong, Su, Zhu: "The inhibitory effect of a new scFv/tP protein as siRNA delivery system to target hWAPL in cervical carcinoma." in: Molecular and cellular biochemistry, Vol. 391, Issue 1-2, pp. 77-84, (2014) (PubMed).

Achmad, Hanaoka, Yoshioka, Yamamoto, Tominaga, Araki, Ohshima, Oriuchi, Endo: "Predicting cetuximab accumulation in KRAS wild-type and KRAS mutant colorectal cancer using 64Cu-labeled cetuximab positron emission tomography." in: Cancer science, Vol. 103, Issue 3, pp. 600-5, (2012) (PubMed).

Qin Li, Huang, Ping, Xu, Li, Dai: "Expression of midkine and endoglin in breast carcinomas with different immunohistochemical profiles." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 119, Issue 2, pp. 103-10, (2011) (PubMed).

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." in: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, (2009) (PubMed).

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background:

Background: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Gene Full Name: epidermal growth factor receptor

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins