VCAM1 ELISA Kit

Catalog No. ABIN411367

Human VCAM1 ELISA Kit, Colorimetric assay for quantification of Human VCAM1, that has been mentioned in 18+ publications.

Quick Overview for VCAM1 ELISA Kit (ABIN411367)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Binding Specificity: AA 25-698

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details VCAM1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human VCAM-1 ELISA Kit to quantitate Human VCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: F25-E698

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.7%, 4.8%, 7.3%
Inter-Assay Precision (CV%): 6.4%, 6.4%, 8.6%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for VCAM1 ELISA Kit (ABIN411367)

Dehghan, Sabri, Javanmard, Ahmadi, Mansourian: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).

Sun, Chen, Wang, Wan, Zhang, Zhang, Lin, Zhang: "Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6905217, (2018) (PubMed).

Xu, Liang, Li, Ma: "Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer." in: Oncology letters, Vol. 15, Issue 6, pp. 9293-9298, (2018) (PubMed).

Liao, Cai, Xu, Wang, Qiu, Xie, Huang, Sui: "Protective Role of Antioxidant Huskless Barley Extracts on TNF-α-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3846029, (2018) (PubMed).

Maggi, Bruno, Perilli, Saracino, Volpe, Santoro, Ladisa, Angarano: "Effects of Therapy with Maraviroc on the Carotid Intima Media Thickness in HIV-1/HCV Co-infected Patients." in: In vivo (Athens, Greece), Vol. 31, Issue 1, pp. 125-131, (2017) (PubMed).

Sun, Zhu, Cai, Qiu: "Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway." in: International journal of molecular sciences, Vol. 18, Issue 4, (2017) (PubMed).

Denkovskij, Bagdonas, Kusleviciute, Mackiewicz, Unguryte, Porvaneckas, Fleury, Venalis, Jorgensen, Bernotiene: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." in: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).

Llauradó, Megia, Cano, Giménez-Palop, Simón, González-Sastre, Berlanga, Fernández-Veledo, Vendrell, González-Clemente: "FGF-23/Vitamin D Axis in Type 1 Diabetes: The Potential Role of Mineral Metabolism in Arterial Stiffness." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140222, (2016) (PubMed).

Asgary, Soltani, Mirvakili, Sarrafzadegan: "Evaluation of the effect of Vaccinium arctostaphylos L. fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial." in: Research in pharmaceutical sciences, Vol. 11, Issue 4, pp. 343-8, (2016) (PubMed).

Majidinia, Rasmi, Khadem Ansari, Seyed-Mohammadzad, Saboory, Shirpoor: "Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X." in: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 3, pp. 561-566, (2016) (PubMed).

Barros, Borges, Ferreira, Carmo, Rosado, Fouque, Mafra: "Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?" in: Future microbiology, Vol. 10, Issue 4, pp. 517-26, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Sabri, Dehghan, Yaghini, Nasiri, Mansourian, Khalifehsoltani: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." in: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." in: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Asgary, Kelishadi, Rafieian-Kopaei, Najafi, Najafi, Sahebkar: "Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents." in: Pediatric cardiology, Vol. 34, Issue 7, pp. 1729-35, (2013) (PubMed).

Zhang, Zheng, Shu, Liu, Zhang, Liu, Bai, Hu, Mao, Wang: "Human astrocytic cells support persistent coxsackievirus B3 infection." in: Journal of virology, Vol. 87, Issue 22, pp. 12407-21, (2013) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: VCAM1

Background:

Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

Gene Full Name: vascular cell adhesion molecule 1

Gene ID: 7412

UniProt: P19320

Pathways: Carbohydrate Homeostasis