Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

VEGF ELISA Kit

VEGF Reactivity: Rat AA 27-190 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Catalog No. ABIN411370
  • Target See all VEGF ELISA Kits
    VEGF (Vascular Endothelial Growth Factor (VEGF))
    Binding Specificity
    AA 27-190
    Reactivity
    • 8
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat VEGF
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO,A27-R190
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <1pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A27-R190
    Featured
    Discover our best selling VEGF ELISA Kit
    Top Product
    Discover our top product VEGF ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    rat VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF has been precoated onto 96-well plates. Standards (NSO,A27-R190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat VEGF amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL rat VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat VEGF standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 120, Standard deviation: 4.44, CV(%): 3.7
    • Sample 2: n=16, Mean(pg/ml): 305, Standard deviation: 15.86, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 29.81, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 236, Standard deviation: 10.62, CV(%): 4.5
    • Sample 2: n=24, Mean(pg/ml): 289, Standard deviation: 18.21, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 632, Standard deviation: 34.76, CV(%): 5.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Zhou, Tan, Wang, Wang: "Hypoxic preconditioning-induced autophagy enhances survival of engrafted endothelial progenitor cells in ischaemic limb." in: Journal of cellular and molecular medicine, Vol. 21, Issue 10, pp. 2452-2464, (2018) (PubMed).

    Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

    Ling, Feng, Wei, Wang, Wang, Zhang, He, Wang, Zeng, Xiong: "Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 283, (2018) (PubMed).

    Machado, Rodrigues-Baptista, Alessandra-Perini, Soares de Moura, Santos, Pereira, Marinho da Silva, Souza, Nasciutti, Perini: "Euterpe oleracea Extract (Açaí) Is a Promising Novel Pharmacological Therapeutic Treatment for Experimental Endometriosis." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166059, (2017) (PubMed).

    Gong, Yuan, Liu, Qi: "Investigation of the Effects and Mechanisms of Mai Tong Formula on Lower Limb Macroangiopathy in a Spontaneous Diabetic Rat Model." in: Journal of diabetes research, Vol. 2016, pp. 8076796, (2017) (PubMed).

    Peng, Xie, Wang, Wu, Liu, Wang, Ma, Chang, Yang: "MOXIBUSTION ALLEVIATES GASTRIC PRECANCEROUS LESIONS IN RATS BY PROMOTING CELL APOPTOSIS AND INHIBITING PROLIFERATION-RELATED ONCOGENES." in: African journal of traditional, complementary, and alternative medicines : AJTCAM, Vol. 14, Issue 2, pp. 148-160, (2017) (PubMed).

    Dong, Zhong, Chen, Wu, Zhang, Huang, Yang: "Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3647080, (2017) (PubMed).

    Li, Wu, Chen, Chen, Xu, Gu, Song: "Antiplatelet drug ticagrelor delays gastric ulcer healing in rats." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3774-3779, (2017) (PubMed).

    Rong, Sang, Qian, Meng, Zhao, Li: "Biocompatibility of porcine small intestinal submucosa and rat endothelial progenitor cells in vitro." in: International journal of clinical and experimental pathology, Vol. 8, Issue 2, pp. 1282-91, (2016) (PubMed).

    Jin, Wang, Wang, Long, Wang: "Brain protection against ischemic stroke using choline as a new molecular bypass treatment." in: Acta pharmacologica Sinica, Vol. 36, Issue 12, pp. 1416-25, (2016) (PubMed).

    Chen, Cao, Ma, Wang, Chen, Yan, Zhao, Geng, Wang: "Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways." in: Scientific reports, Vol. 6, pp. 20853, (2016) (PubMed).

    Sancakdar, Guven, Uysal, Deveci, Gültürk: "Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean-Congo Hemorrhagic Fever." in: The Pediatric infectious disease journal, Vol. 34, Issue 8, pp. e200-5, (2015) (PubMed).

    Fan, Zhang, Xu, Ke, Ding, Wang, Liu, Ning, Deng, He: "Serum level of vascular endothelial growth factor decreased in chronic ketamine abusers." in: Drug and alcohol dependence, Vol. 152, pp. 57-61, (2015) (PubMed).

    Das, Jargar, Saha, Yendigeri, Singh: "?-tocopherol supplementation prevents lead acetate and hypoxia-induced hepatic dysfunction." in: Indian journal of pharmacology, Vol. 47, Issue 3, pp. 285-91, (2015) (PubMed).

    Zhang, Chen, Fan, Wang, Zhu: "Overexpression of VEGF183 promotes murine breast cancer cell proliferation in vitro and induces dilated intratumoral microvessels." in: Tumour biology, Vol. 36, Issue 5, pp. 3871-80, (2015) (PubMed).

    Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

    Chen, Xu, Li, Du, Chen, Liu, Peng, Wang, Song: "Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress." in: International immunopharmacology, Vol. 26, Issue 1, pp. 147-52, (2015) (PubMed).

    Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

    Dayi, Cetin, Sisman, Aksu, Tas, Gönenc, Uysal: "The effects of oxytocin on cognitive defect caused by chronic restraint stress applied to adolescent rats and on hippocampal VEGF and BDNF levels." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 69-75, (2015) (PubMed).

    Liu, Zhang, Xiao, Dai, Sun, Chen, Chen: "Three-dimensional collagen scaffold enhances the human adenoid cystic carcinoma cancer stem cell and epithelial-mesenchymal transition properties." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 4, pp. 772-80, (2014) (PubMed).

  • Target See all VEGF ELISA Kits
    VEGF (Vascular Endothelial Growth Factor (VEGF))
    Abstract
    VEGF Products
    Synonyms
    MVCD1 ELISA Kit, VEGF ELISA Kit, VPF ELISA Kit, Vegf ELISA Kit, Vegf120 ELISA Kit, Vegf164 ELISA Kit, Vegf188 ELISA Kit, Vpf ELISA Kit, VEGF-A ELISA Kit, VEGF164 ELISA Kit, eVEGF120 ELISA Kit, eVEGF164 ELISA Kit, vegf-a ELISA Kit, vegf ELISA Kit, vascular endothelial growth factor A ELISA Kit, vascular endothelial growth factor A L homeolog ELISA Kit, vascular endothelial growth factor ELISA Kit, vascular endothelial growth factor precursor ELISA Kit, VEGFA ELISA Kit, Vegfa ELISA Kit, vegfa.L ELISA Kit, VEGF ELISA Kit, vegf ELISA Kit
    Background

    Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.

    Synonyms: Vascular endothelial growth factor A ,Vegfa ,

    Full Gene Name: vascular endothelial growth factor A

    Gene ID
    83785
    UniProt
    B5DEK7
You are here:
Support