FLT1 ELISA Kit

Catalog No. ABIN411372

The Human FLT1 ELISA Kit (ABIN411372) is a Colorimetric ELISA Kit designed to quantify Human FLT1. This ELISA Kit has been cited in 3 publications.

Quick Overview for FLT1 ELISA Kit (ABIN411372)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Binding Specificity: AA 27-687

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA)

Product Details FLT1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human VEGFR1/FLT1 ELISA Kit to quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: S27-H687

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 30 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.1%, 7.8%, 4.6%
Inter-Assay Precision (CV%): 4.7%, 9.7%, 4.7%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for FLT1 ELISA Kit (ABIN411372)

Bozduman, Ersoy Evans, Karahan, Hayran, Akbiyik, Lay: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." in: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).

Bakir, Bakir, Sari, Celik, Gozel, Engin: "Evaluation of the relationship between serum levels of VEGF and sVEGFR1 with mortality and prognosis in patients with Crimean-Congo hemorrhagic fever." in: Journal of medical virology, Vol. 85, Issue 10, pp. 1794-801, (2013) (PubMed).

Liu, Li, Su, Huang, Luo: "Soluble Fms-like tyrosine kinase-1 expression inhibits the growth of multiple myeloma in nude mice." in: Acta biochimica et biophysica Sinica, Vol. 39, Issue 7, pp. 499-506, (2007) (PubMed).

Target Details for FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: FLT1

Background:

Background: Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Gene Full Name: fms related receptor tyrosine kinase 1

Gene ID: 2321

UniProt: P17948

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals