Angiopoietin 1 ELISA Kit
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- Target See all Angiopoietin 1 (ANGPT1) ELISA Kits
- Angiopoietin 1 (ANGPT1)
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78-50 ng/mL
- Minimum Detection Limit
- 0.78 ng/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of mouse Angiopoietin 1,ANG-1 concentrations in cell culture supernates, serum, plasma and other biological fluids.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay recognizes recombinant and natural mouse ANG-1.
- Cross-Reactivity (Details)
- No significant cross-reactivity or interference was observed.
- Sensitivity
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
- Characteristics
- Mus musculus,Mouse,Angiopoietin-1,ANG-1,Angpt1,Agpt
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- Discover our best selling ANGPT1 ELISA Kit
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- Discover our top product ANGPT1 ELISA Kit
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANG-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANG-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANG-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
- Storage Comment
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Target See all Angiopoietin 1 (ANGPT1) ELISA Kits
- Angiopoietin 1 (ANGPT1)
- Alternative Name
- Angpt1 (ANGPT1 Products)
- Synonyms
- AGP1 ELISA Kit, AGPT ELISA Kit, ANG1 ELISA Kit, 1110046O21Rik ELISA Kit, Ang-1 ELISA Kit, Ang1 ELISA Kit, Agpt ELISA Kit, Agpt1 ELISA Kit, ang1 ELISA Kit, wu:fc76g06 ELISA Kit, ANG-1 ELISA Kit, angpt1 ELISA Kit, MGC80788 ELISA Kit, angiopoietin 1 ELISA Kit, angiopoietin 1 S homeolog ELISA Kit, ANGPT1 ELISA Kit, Angpt1 ELISA Kit, angpt1 ELISA Kit, angpt1.S ELISA Kit
- Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process
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