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Angiopoietin 1 ELISA Kit

ANGPT1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN454256
  • Target See all Angiopoietin 1 (ANGPT1) ELISA Kits
    Angiopoietin 1 (ANGPT1)
    Reactivity
    • 11
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78-50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Purpose
    This immunoassay kit allows for the in vitro quantitative determination of mouse Angiopoietin 1,ANG-1 concentrations in cell culture supernates, serum, plasma and other biological fluids.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay recognizes recombinant and natural mouse ANG-1.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference was observed.
    Sensitivity
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Characteristics
    Mus musculus,Mouse,Angiopoietin-1,ANG-1,Angpt1,Agpt
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANG-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANG-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANG-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
    Storage Comment
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Target See all Angiopoietin 1 (ANGPT1) ELISA Kits
    Angiopoietin 1 (ANGPT1)
    Alternative Name
    Angpt1 (ANGPT1 Products)
    Synonyms
    AGP1 ELISA Kit, AGPT ELISA Kit, ANG1 ELISA Kit, 1110046O21Rik ELISA Kit, Ang-1 ELISA Kit, Ang1 ELISA Kit, Agpt ELISA Kit, Agpt1 ELISA Kit, ang1 ELISA Kit, wu:fc76g06 ELISA Kit, ANG-1 ELISA Kit, angpt1 ELISA Kit, MGC80788 ELISA Kit, angiopoietin 1 ELISA Kit, angiopoietin 1 S homeolog ELISA Kit, ANGPT1 ELISA Kit, Angpt1 ELISA Kit, angpt1 ELISA Kit, angpt1.S ELISA Kit
    Pathways
    RTK Signaling, Glycosaminoglycan Metabolic Process
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