Aquaporin 4 ELISA Kit

Catalog No. ABIN454459

Mouse Aquaporin 4 ELISA Kit, Colorimetric assay for quantification of Mouse Aquaporin 4.

Quick Overview for Aquaporin 4 ELISA Kit (ABIN454459)

Target: Aquaporin 4 (AQP4)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details Aquaporin 4 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse AQP-4 concentrations in tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse AQP-4.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.039 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Aquaporin-4,AQP-4,Mercurial-insensitive water channel,MIWC,WCH4,Aqp4

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of AQP-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: Aqp4

Background: Aquaporin-4 (AQP4), is a channel protein that allows water to move in and out of cells. Aquaporin-4 is located in many tissues, including the basolateral membrane of the renal cortical and medullary principal cell, as well as in the brain.Aquaporin-4 is the first specific molecule to be defined as a target for the autoimmune response in any form of multiple sclerosis. Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.

Pathways: Sensory Perception of Sound