GFAP ELISA Kit
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- Target See all GFAP ELISA Kits
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2-2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Mouse Glial Fibrillary Acidic Protein(GFAP) concentrations in cell culture supernates, serum, plasma, tissue homogenates and other biological fluids.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay recognizes recombinant and natural mouse GFAP.
- Cross-Reactivity (Details)
- No significant cross-reactivity or interference was observed.
- Sensitivity
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< 15.5 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - Characteristics
- Mus musculus,Mouse,Glial fibrillary acidic protein,GFAP,Gfap
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- Discover our best selling GFAP ELISA Kit
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- Discover our top product GFAP ELISA Kit
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GFAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
- Storage Comment
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Target See all GFAP ELISA Kits
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Alternative Name
- Gfap (GFAP Products)
- Synonyms
- GFAP ELISA Kit, AI836096 ELISA Kit, cb345 ELISA Kit, etID36982.3 ELISA Kit, gfapl ELISA Kit, wu:fb34h11 ELISA Kit, wu:fk42c12 ELISA Kit, xx:af506734 ELISA Kit, zgc:110485 ELISA Kit, glial fibrillary acidic protein ELISA Kit, GFAP ELISA Kit, LOC100136168 ELISA Kit, gfap ELISA Kit, Gfap ELISA Kit
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