GFAP ELISA Kit

Catalog No. ABIN455197

Mouse GFAP ELISA Kit, Colorimetric assay for quantification of Mouse GFAP.

Quick Overview for GFAP ELISA Kit (ABIN455197)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Product Details GFAP ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Mouse Glial Fibrillary Acidic Protein(GFAP) concentrations in cell culture supernates, serum, plasma, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse GFAP.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 15.5 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Glial fibrillary acidic protein,GFAP,Gfap

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GFAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: Gfap