Interferon gamma ELISA Kit

Catalog No. ABIN455496

The Mouse Interferon gamma ELISA Kit (ABIN455496) is a Colorimetric ELISA Kit designed to quantify Mouse Interferon gamma.

Quick Overview for Interferon gamma ELISA Kit (ABIN455496)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse IFN- ? concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse IFN- ? .

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Interferon gamma,IFN-gamma,Ifng

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN- γ . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFN- γ . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFN- γ , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a 2 change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFN- γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: Ifng

Background: IFN- γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor. The IFN- γ monomer consists of a core of six α -helices and an extended unfolded sequence in the C-terminal region. The biologically active dimer is formed by anti-parallel inter-locking of two monomers. Full length of IFN- γ is 143 amino acids. In contrast to interferon- α and interferon- β which can be expressed by all cells, IFN- γ is secreted by T lymphocytes and NK cells only. Also known as immune interferon, IFN- γ is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. IFN- γ has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by IFN- γ is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). IFN- γ binding to the receptor activates the JAK-STAT pathway. In addition, IFN- γ activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. IFN- γ is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and [[CD8+ cytotoxic T cell]]s also produce IFN- γ . IFN- γ suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NF κ B.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy