IL12A ELISA Kit

Catalog No. ABIN455530

Product Details IL12A ELISA Kit

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Purpose: This immunoassay kit allows for the specific measurement of Mouse Interleukin,IL-12 concentrations in cell culture supernates, serum and plasma.

Sample Type: Cell Culture Supernatant, Serum, Plasma

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural Mouse IL-12.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Characteristics: Mus musculus,Mouse,Interleukin-12 subunit alpha,IL-12A,Cytotoxic lymphocyte maturation factor 35 kDa subunit,CLMF p35,IL-12 subunit p35,Il12a

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. An 2 enzyme-linked polyclonal antibody specific for IL-12 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Il12a (IL12A Products)

Synonyms: CLMF ELISA Kit, IL-12A ELISA Kit, NFSK ELISA Kit, NKSF1 ELISA Kit, P35 ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12p35 ELISA Kit, Il-12a ELISA Kit, Ll12a ELISA Kit, p35 ELISA Kit, IL12p35 ELISA Kit, Il-12 p35 ELISA Kit, IL12p40 ELISA Kit, IL12 ELISA Kit, IL-12 p35 ELISA Kit, interleukin 12A ELISA Kit, interleukin 12B ELISA Kit, interleukin 12b ELISA Kit, interleukin 12a ELISA Kit, Interleukin 12 level ELISA Kit, IL12A ELISA Kit, IL12B ELISA Kit, Il12b ELISA Kit, Il12a ELISA Kit, IL12 ELISA Kit, il12a ELISA Kit

Background: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of cultured EBV-transformed RPMI-8866 cells . IL-12 is a 75 kDa glycoprotein heterodimer composed of two genetically unrelated subunits linked by a disulfide bond. The smaller subunit (p35) has homology to IL-6 and G-CSF while the larger subunit (p40) demonstrates similarity to the soluble receptor for IL-6, leading to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex . Each subunit of IL-12 apparently arises from a single copy gene. The mRNA transcription of the subunits is closely coordinated, although an excess of the larger subunit has been shown to be produced by B cells in addition to active IL-12 . Expression of p35 is reported to be enhanced by simultaneous expression of p40. IL-12 activity cannot be demonstrated in the absence of either chain . As suggested by their names, p35 has a native molecular weight of 35 kDa while p40 has a native molecular weight of 40 kDa. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells . These include inducing production of IFN-gama and TNF by resting and activated T and NK cells, synergizing with other IFN-gama inducers at both the transcriptional and post-transcriptional levels to induce IFN- gama gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a comitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells . Evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells . These activities of IL-12 are antagonized by IL-4 and IL-10, factors associated with the development of uncommitted T helper cells into TH2 cells and mediation of the humoral immune response .

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation