IL-18 ELISA Kit

Catalog No. ABIN455546

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-18.

Quick Overview for IL-18 ELISA Kit (ABIN455546)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL-18 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 18,IL-18 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse IL-18.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Interleukin-18,IL-18,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,Il18,Igif

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-18. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-18 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Il18

Background: Interleukin 18 (IL-18) is a 18 kDa novel cytokine which is identified as a costimulatory factor for production of interferon- γ (IFN- γ ) in response to toxic shock. It shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 ß converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It has been reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 acts on T helper 1-type T (Th1) cells and in combination with IL-12 strongly induces production of IFN- γ by these cells. Pleiotropic effects of IL-18 have also been reported, including enhancement production of IFN- γ and GM-CSF in peripheral blood mononuclear cells, production of T helper type 1 cytokines, IL-2, GM-CSF and IFN- γ in T cells, enhancement of Fas ligand expression by T helper type 1 cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome