IL-2 ELISA Kit

Catalog No. ABIN455562

The Mouse IL-2 ELISA Kit (ABIN455562) is a Colorimetric ELISA Kit designed to quantify Mouse IL-2.

Quick Overview for IL-2 ELISA Kit (ABIN455562)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL-2 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 2, IL-2 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse IL-2.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Interleukin-2,IL-2,T-cell growth factor,TCGF,Il2,Il-2

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Il2

Background: Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.IL-2 stimulates the proliferation of thymocytes, stimulates the proliferation and differentiation of activated B cells, promotes the growth, differentiation and cytocidal activity of monocytes, induces the growth of and cytokine production and cytolytic activity by natural killer cells, enhances the production of lymphocyte-activated killer (LAK) cells, and induces the proliferation and differentiation of oligodendrocytes . Interleukin 2,IL-2 is a protein of 133 amino acids (15.4 kDa). It is produced mainly by T-cells expressing the surface antigen CD4 following cell activation by mitogens or allogens under physiological conditions. IL-2 displays significant anti-tumor activity for a variety of tumor cell types since it supports the proliferation and clonal expansion of T-cells that specifically attack certain tumor types.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation