LIF ELISA Kit

Catalog No. ABIN455685

The Human LIF ELISA Kit (ABIN455685) is a Colorimetric ELISA Kit designed to quantify Human LIF.

Quick Overview for LIF ELISA Kit (ABIN455685)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma

Product Details LIF ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: This immunoassay kit allows for the specific measurement of human LIF concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human LIF.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Characteristics: Homo sapiens,Human,Leukemia inhibitory factor,LIF,Differentiation-stimulating factor,D factor,Melanoma-derived LPL inhibitor,MLPLI,LIF,HILDA

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for LIF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Leukemia Inhibitory Factor (or LIF) is a variably glycosylated, 38 - 67 kDa polypeptide originally identified as a proliferation inhibitor and differentiation inducer of the mouse M1 myeloid leukemia cell line. The mature LIF molecule measures 180 amino acid (aa) residues in length, with multiple potential N-linked and O-linked glycosylation sites plus six conserved cysteines that form three intramolecular disulfide bridges. Mature mouse LIF is 78% identical to human LIF at the aa sequence level. Although such homology might suggest a high conservation of LIF biology, notable differences exist between the reported forms of mouse and human LIF proteins and their receptors. For example, alternative splice events are known to occur in mice, but not humans, creating two isoforms of secreted LIF. Also, three isoforms of the LIF receptor a-chain, two soluble forms and one transmembrane form, have been reported in mice, but not in humans. Based on its helical structure, LIF is considered to be a member of the Interleukin-6 family of cytokines. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts , keratinocytes, regenerating skeletal muscle, mast cells , and fibroblasts.

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding