AGLU ELISA Kit

Catalog No. ABIN455810

Product Details AGLU ELISA Kit

Target: AGLU (alpha-Glucosidase (AGLU))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human MAL concentrations in tissue homogenates and other biological fluids.

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human MAL.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.78 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Maltase-glucoamylase, intestinal,MGAM,MGA,MGAML

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for AGLU

Target: AGLU (alpha-Glucosidase (AGLU))

Alternative Name: MGAM (AGLU Products)

Synonyms: MG ELISA Kit, MGA ELISA Kit, SPAC56F8.01 ELISA Kit, SPAC922.02c ELISA Kit, CG11909 ELISA Kit, CT33098 ELISA Kit, Dmel\\CG11909 ELISA Kit, GB19017 ELISA Kit, 6030407P20Rik ELISA Kit, maltase-glucoamylase ELISA Kit, alpha-glucosidase (predicted) ELISA Kit, alpha-glucosidase ELISA Kit, glucosidase, alpha, acid ELISA Kit, target of brain insulin ELISA Kit, sucrase-isomaltase ELISA Kit, MGAM ELISA Kit, SPAC30D11.01c ELISA Kit, LMOf2365_0194 ELISA Kit, SPAC1039.11c ELISA Kit, Gaa ELISA Kit, tobi ELISA Kit, SI ELISA Kit, Hbg3 ELISA Kit, Mgam ELISA Kit

Background: Maltase (acid maltase, alpha-1,4-glucosidase) is an enzyme produced by the cells lining the small intestine that breaks down the disaccharide maltose. It comes under the enzyme category carbohydrase (which is a subcategory of hydrolase). Maltase is secreted by the surface cells of the villi, which are thin projections on the mucosa. These are found throughout the small intestine, but differ in shape in the duodenum and ileum sections. It must also be located in other places, like the heart, if a deficiency of it causes Pompe's Disease (which results in cardiomegaly).

Pathways: Cellular Glucan Metabolic Process