Prolactin ELISA Kit

Catalog No. ABIN456250

Mouse Prolactin ELISA Kit, Colorimetric assay for quantification of Mouse Prolactin.

Quick Overview for Prolactin ELISA Kit (ABIN456250)

Target: Prolactin (PRL)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Prolactin ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse prolactin, PRL concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse prolactin.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Prolactin,PRL,Prl

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to prolactin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for prolactin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain prolactin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Prolactin

Target: Prolactin (PRL)

Alternative Name: Prl

Background: Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of prolactin is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition factors.

Pathways: JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus