F2RL1 ELISA Kit

Catalog No. ABIN456689

This Colorimetric ELISA kit is designed for the quantitative measurement of Human F2RL1.

Quick Overview for F2RL1 ELISA Kit (ABIN456689)

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Serum, Tissue Homogenate

Product Details F2RL1 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human TPS, concentrations in serum, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human TPS.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.036 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Proteinase-activated receptor 2,PAR-2,Coagulation factor II receptor-like 1,G-protein coupled receptor 11,Thrombin receptor-like 1,F2RL1,GPR11,PAR2

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TPS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for F2RL1

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Alternative Name: F2RL1

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome