PLAU ELISA Kit

Catalog No. ABIN456751

Human PLAU ELISA Kit, Colorimetric assay for quantification of Human PLAU.

Quick Overview for PLAU ELISA Kit (ABIN456751)

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details PLAU ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human prolactin concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: 5 This assay recognizes recombinant and natural human prolactin.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.039 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Urokinase-type plasminogen activator,U-plasminogen activator,uPA,PLAU,3.4.21.73

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to prolactin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for prolactin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain prolactin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for PLAU

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: PLAU

Background: UPA is a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. For example, the combination (both low vs. either or both high) of uPA and its inhibitor, plasminogen activator inhibitor-1 (PAI-1), outperforms the single factors as well as other traditional prognostic factors with regard to risk group assessment for breast cancer, particularly in node-negative breast cancer. The uPA is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule. The B chain starting at Ile179 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Ser21 (the long form) and the other at Lys156 (the short form). The resulting two-chain forms have different molecular weights (MW). The B chain is common for both forms whereas the long and short A chains are unique to the high and low MW forms, respectively. The long A chain contains an EGF-like domain, which is responsible for binding of the uPA receptor (uPAR). Both high and low MW forms exist in the purified recombinant Rabbit uPA.

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration