ITLN1/Omentin ELISA Kit

Catalog No. ABIN4881321

Human, Rat and Mouse ITLN1/Omentin ELISA Kit, Colorimetric assay for quantification of Human, Rat and Mouse ITLN1/Omentin, that has been mentioned in 1 publication.

Quick Overview for ITLN1/Omentin ELISA Kit (ABIN4881321)

Target: ITLN1/Omentin (ITLN1) (Intelectin 1 (Galactofuranose Binding) (ITLN1))

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details ITLN1/Omentin ELISA Kit

Minimum Detection Limit: 0.1 ng/mL

Purpose: Human/Mouse/Rat Omentin EIA Kit optimized for serum and cell culture medium. Competition-based ELISA

Analytical Method: Quantitative

Specificity: Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, Biotinylated Omentin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

References for ITLN1/Omentin ELISA Kit (ABIN4881321)

Luedde, Benz, Niedeggen, Vucur, Hippe, Spehlmann, Schueller, Loosen, Frey, Trautwein, Koch, Luedde, Tacke, Roderburg: "Elevated Omentin Serum Levels Predict Long-Term Survival in Critically Ill Patients." in: Disease markers, Vol. 2016, pp. 3149243, (2017) (PubMed).

Target Details for ITLN1/Omentin

Target: ITLN1/Omentin (ITLN1) (Intelectin 1 (Galactofuranose Binding) (ITLN1))

Alternative Name: Omentin

Background:

Gene Symbols: ITLN1, INTL, ITLN, LFR, UNQ640/PRO1270

Background: Omentin is a newly identified secretary protein that is highly and selectively expressed in visceral adipose tissue relative to subcutaneous adipose tissue. Omentin has been identified in other tissues at lower expression levels and named intelectin, intestinal lactoferrin receptor, or endothelial lectin. It is expressed in intestinal paneth cells, endothelial cells and visceral adipose stromal -vascular cells. In vitro studies have shown that omentin increases insulin signal transduction by activating the protein kinase Akt/protein kinase B and enhancing insulin-stimulated glucose transport in isolated human adipocytes. Thus, omentin may play a paracrine or endocrine role in modulating insulin sensitivity.

The expression of omentin 1 and its homolog omentin 2 [intelectin-2] has been shown to be decreased with obesity. Omentin 1 is the major circulating form and its plasma levels are decreased in obesity. In other tissues omentin 1 is expressed at lower levels and has originally been shown to be involved in body defense by binding to galactofuranoses on bacteria.

A homolog of omentin has been identified that shares 83 % amino acid identity with omentin/intelectin and will be referred to as omentin 2. The two omentin genes, omentin1 and omentin 2, are located adjacent to each other in the 1q22-q23 chromosomal region, which has been previously linked to type 2 diabetes in several populations.

Protein Name (Synonyms): Intelectin-1 (ITLN-1) (Endothelial lectin HL-1) (Galactofuranose-binding lectin) (Intestinal lactoferrin receptor) (Omentin)

Gene ID: 55600

UniProt: Q8WWA0