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Caspase 8 ELISA Kit

CASP8 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882103
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    Caspase 8 (CASP8)
    Reactivity
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Caspase-8 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human FHL1.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Caspase 8 (CASP8) ELISA Kits
    Caspase 8 (CASP8)
    Alternative Name
    Caspase-8 (CASP8 Products)
    Synonyms
    casp8-A ELISA Kit, xCaspase-8 ELISA Kit, casp8 ELISA Kit, CASP8 ELISA Kit, CASP-8 ELISA Kit, FLICE ELISA Kit, MACH ELISA Kit, Mch5 ELISA Kit, ALPS2B ELISA Kit, CAP4 ELISA Kit, Casp-8 ELISA Kit, MCH5 ELISA Kit, caspase-8 ELISA Kit, zgc:92075 ELISA Kit, caspase 8 L homeolog ELISA Kit, death related ced-3/Nedd2-like protein ELISA Kit, caspase 8 ELISA Kit, caspase-8 ELISA Kit, caspase-8-like ELISA Kit, caspase 8, apoptosis-related cysteine peptidase ELISA Kit, casp8.L ELISA Kit, LOC661095 ELISA Kit, CASP8 ELISA Kit, LOC5563818 ELISA Kit, casp8 ELISA Kit, LOC100222284 ELISA Kit, Casp8 ELISA Kit
    Gene ID
    841
    UniProt
    Q14790
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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