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LGALS1/Galectin 1 ELISA Kit
LGALS1
Reactivity: Human
Colorimetric
Sandwich ELISA
4.10-1.000 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details LGALS1/Galectin 1 ELISA Kit
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Target
See all LGALS1/Galectin 1 (LGALS1) ELISA Kits
LGALS1/Galectin 1 (LGALS1)
(Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
Reactivity
All reactivities for LGALS1/Galectin 1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
4.10-1.000 ng/mL
Minimum Detection Limit
4.10 ng/mL
Application
ELISA
Purpose
Human Galectin-1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1, BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1, CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine, GITR Ligand, Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for LGALS1/Galectin 1
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Target
See all LGALS1/Galectin 1 (LGALS1) ELISA Kits
LGALS1/Galectin 1 (LGALS1)
(Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
Alternative Name
Galectin-1 (LGALS1 Products )
Synonyms
GAL1 ELISA Kit, GBP ELISA Kit, Galectin-1 ELISA Kit, LGAS1 ELISA Kit, LGALS1 ELISA Kit, MGC64502 ELISA Kit, GB10026 ELISA Kit, AA410090 ELISA Kit, Gal-1 ELISA Kit, Galbp ELISA Kit, L-14.5 ELISA Kit, L14 ELISA Kit, Lect14 ELISA Kit, galectin-1 ELISA Kit, Galaptin ELISA Kit, galectin 1 ELISA Kit, galectin-1 ELISA Kit, lectin, galactoside-binding, soluble, 1 L homeolog ELISA Kit, protein enabled ELISA Kit, lectin, galactose binding, soluble 1 ELISA Kit, LGALS1 ELISA Kit, Lgals1 ELISA Kit, CC1G_05003 ELISA Kit, CC1G_05004 ELISA Kit, lgals1.L ELISA Kit, LOC408848 ELISA Kit
Gene ID
3956
UniProt
P09382
Pathways
Carbohydrate Homeostasis
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