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ITGB1 ELISA Kit

ITGB1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4883481
  • Target See all ITGB1 ELISA Kits
    ITGB1 (Integrin beta 1 (ITGB1))
    Reactivity
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Integrin beta 1/CD29 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes .
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all ITGB1 ELISA Kits
    ITGB1 (Integrin beta 1 (ITGB1))
    Alternative Name
    Integrin beta 1 (ITGB1 Products)
    Synonyms
    CD29 ELISA Kit, FNRB ELISA Kit, GPIIA ELISA Kit, MDF2 ELISA Kit, MSK12 ELISA Kit, VLA-BETA ELISA Kit, VLAB ELISA Kit, 4633401G24Rik ELISA Kit, AA409975 ELISA Kit, AA960159 ELISA Kit, ENSMUSG00000051907 ELISA Kit, Fnrb ELISA Kit, Gm9863 ELISA Kit, gpIIa ELISA Kit, cd29 ELISA Kit, fnrb ELISA Kit, gpiia ELISA Kit, itgb1 ELISA Kit, mdf2 ELISA Kit, msk12 ELISA Kit, vla-beta ELISA Kit, vlab ELISA Kit, itgb1-a ELISA Kit, itgb1-b ELISA Kit, integrin subunit beta 1 ELISA Kit, integrin beta 1 (fibronectin receptor beta) ELISA Kit, integrin subunit beta 1 S homeolog ELISA Kit, integrin beta 1 ELISA Kit, integrin subunit beta 1 L homeolog ELISA Kit, ITGB1 ELISA Kit, Itgb1 ELISA Kit, itgb1.S ELISA Kit, LOC100009173 ELISA Kit, itgb1.L ELISA Kit
    Gene ID
    3688
    UniProt
    P05556
    Pathways
    Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
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