MCL-1 ELISA Kit

Catalog No. ABIN4883721

Product Details MCL-1 ELISA Kit

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Mcl-1/Bcl2L3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: Mcl-1 (MCL1 Products)

Synonyms: BCL2L3 ELISA Kit, EAT ELISA Kit, MCL1-ES ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, Mcl-1 ELISA Kit, TM ELISA Kit, bcl2-L-3 ELISA Kit, mcl1/EAT ELISA Kit, AW556805 ELISA Kit, mcl-1 ELISA Kit, fb72f02 ELISA Kit, wu:fb70a08 ELISA Kit, wu:fb72f02 ELISA Kit, zfMLP1 ELISA Kit, zfMcl-1a ELISA Kit, zgc:109835 ELISA Kit, zgc:109763 ELISA Kit, E3 ELISA Kit, E3-2 ELISA Kit, RNE3 ELISA Kit, RNU107 ELISA Kit, SNORA63A ELISA Kit, elF-4AII ELISA Kit, MCL1, BCL2 family apoptosis regulator ELISA Kit, myeloid cell leukemia sequence 1 ELISA Kit, MCL1, BCL2 family apoptosis regulator a ELISA Kit, MCL1, BCL2 family apoptosis regulator b ELISA Kit, small nucleolar RNA, H/ACA box 63 ELISA Kit, MCL1 ELISA Kit, Mcl1 ELISA Kit, mcl1a ELISA Kit, mcl1b ELISA Kit, SNORA63 ELISA Kit

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling