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PCK1 ELISA Kit
PCK1
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details PCK1 ELISA Kit
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Target
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PCK1
(phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
Reactivity
All reactivities for PCK1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human PCK1/PEPCK-C ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PCK1
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Target
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PCK1
(phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
Alternative Name
PCK1 (PCK1 Products )
Synonyms
PEPCK-C ELISA Kit, PEPCK1 ELISA Kit, PEPCKC ELISA Kit, PEPCK ELISA Kit, PEPCK-M ELISA Kit, PEPCK2 ELISA Kit, 1810010O14Rik ELISA Kit, 9130022B02Rik ELISA Kit, cb856 ELISA Kit, cb924 ELISA Kit, fj93h11 ELISA Kit, zgc:63869 ELISA Kit, wu:fc51c05 ELISA Kit, wu:fj93h11 ELISA Kit, pepck-c ELISA Kit, pepck1 ELISA Kit, pepckc ELISA Kit, PCK1 ELISA Kit, PPCK1 ELISA Kit, AI265463 ELISA Kit, Pck-1 ELISA Kit, GTP ELISA Kit, PCK ELISA Kit, Pepck ELISA Kit, RATPEPCK ELISA Kit, Ppc1C ELISA Kit, PHOSPHOENOLPYRUVATE CARBOXYKINASE ELISA Kit, T28I19.150 ELISA Kit, T28I19_150 ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, 143299_at ELISA Kit, CG10924 ELISA Kit, CG17725 ELISA Kit, Dmel\\CG17725 ELISA Kit, Dromel_CG17725_FBtr0086701_pepck_mORF ELISA Kit, dPEPCK ELISA Kit, pepck ELISA Kit, PEPC ELISA Kit, PEPCase ELISA Kit, ppc ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, phosphoenolpyruvate carboxykinase 2, mitochondrial ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) ELISA Kit, phosphoenolpyruvate carboxykinase 1 (soluble) ELISA Kit, phosphoenolpyruvate carboxykinase 1 S homeolog ELISA Kit, phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit, phosphoenolpyruvate carboxykinase 1, cytosolic ELISA Kit, phosphoenolpyruvate carboxylase 7 ELISA Kit, Phosphoenolpyruvate carboxykinase ELISA Kit, phosphoenolpyruvate carboxylase ELISA Kit, PCK1 ELISA Kit, PCK2 ELISA Kit, Pck2 ELISA Kit, pck1 ELISA Kit, pck1.S ELISA Kit, LOC100634531 ELISA Kit, Pck1 ELISA Kit, pep7 ELISA Kit, Pepck ELISA Kit, LOC107777405 ELISA Kit
Gene ID
5105
UniProt
P35558
Pathways
Positive Regulation of Peptide Hormone Secretion , Carbohydrate Homeostasis
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