POR ELISA Kit

Catalog No. ABIN4884215

Product Details POR ELISA Kit

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human POR/P450R ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for POR

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Alternative Name: POR (POR Products)

Synonyms: CPR ELISA Kit, CYPOR ELISA Kit, P450R ELISA Kit, POR ELISA Kit, CCR ELISA Kit, CG11567 ELISA Kit, DMR ELISA Kit, DmCPR ELISA Kit, Dmel\\CG11567 ELISA Kit, NCPR ELISA Kit, P450 ELISA Kit, cpr ELISA Kit, zgc:110032 ELISA Kit, npr ELISA Kit, xpor ELISA Kit, 4933424M13Rik ELISA Kit, PORCINO ELISA Kit, T5J17.90 ELISA Kit, T5J17_90 ELISA Kit, TFC C ELISA Kit, TUBULIN-FOLDING COFACTOR C ELISA Kit, cytochrome p450 oxidoreductase ELISA Kit, P450 (cytochrome) oxidoreductase ELISA Kit, ADP-ribosylation factor interacting protein 2 ELISA Kit, Cytochrome P450 reductase ELISA Kit, P450 (cytochrome) oxidoreductase a ELISA Kit, P450 (cytochrome) oxidoreductase L homeolog ELISA Kit, NADPH--cytochrome P450 reductase ELISA Kit, C-CAP/cofactor C-like domain-containing protein ELISA Kit, POR ELISA Kit, Arfip2 ELISA Kit, Por ELISA Kit, Cpr ELISA Kit, pora ELISA Kit, por.L ELISA Kit, LOC100406157 ELISA Kit, por ELISA Kit, LOC100619047 ELISA Kit

Gene ID: 5447

UniProt: P16435

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome